方法一（不使用模块，by agonyr）
#!/usr/bin/perl -w
use strict;
my @seq = ( "A", "T", "C", "G" );
my $length = 10000;
undef my %hash;
$hash{"A"} = int( $length * 0.3 );
$hash{"C"} = int( $length * 0.3 );
$hash{"G"} = int( $length * 0.2 );
$hash{"T"} = int( $length * 0.2 );
my $i = 0;
while ( $i 《 $length ) {
my $word = $seq[ rand(@seq) ];
if ( $hash{$word} ) {
print "$word";
$i++;
}
$hash{$word}--;
}
print "n";
方法二（使用模块，by yixf）
#!/usr/bin/perl
use strict;
use warnings;
use BioX::SeqUtils::RandomSequence;
my $randomizer = BioX::SeqUtils::RandomSequence-》new(
{
l =》 10000,
s =》 1,
y =》 "dna",
a =》 3,
c =》 3,
g =》 2,
t =》 2
}
);
print $randomizer-》rand_seq(), "n";
两种方法比较
设定长度为10000，ACGT的比例为3:3:2:2。
withoutModule Length=10000 GC=49.42% A=2558,C=2503,G=2439,T=2500,Others=0
withModule Length=10000 GC=50.00% A=3000,C=3000,G=2000,T=2000,Others=0